Point spread function of optical microscopes imaging through stratified media

The point spread function of optical microscopes imaging through stratified media.

We propose a model for imaging point objects through a dielectric interface or stratified media. The model is applicable to conventional and confocal fluorescence microscopy, with single- or multiphoton excitation. An analytical solution is obtained in the form of readily computable functions. When large mismatches occur in the refractive indices of the media of the objective lens and specimen ...

Enhancement of Point Spread Function of Planar Fluorescent Imaging System Using Iterative Deconvolution Method

This article has no abstract.

Vectorial point spread function and optical transfer function in oblique plane imaging.

Oblique plane imaging, using remote focusing with a tilted mirror, enables direct two-dimensional (2D) imaging of any inclined plane of interest in three-dimensional (3D) specimens. It can image real-time dynamics of a living sample that changes rapidly or evolves its structure along arbitrary orientations. It also allows direct observations of any tilted target plane in an object of which orie...

Point spread function of the optical needle super-oscillatory lens

Experimental point spread function of fm pulse imaging scheme.

In this paper, we have examined the possibility of incorporating pulse compression techniques into a conventional medical B-scan imaging scheme. Linear frequency modulation fm, one form of pulse coding among many others, has been used in this study. With this approach, one can overcome current peak intensity limitations. A theoretical framework that includes medium propagation effects, transduc...